Synthesis,algal inhibition activities and QSAR studies of novel gramine compounds containing ester functional groups     

李霞  于良民  姜晓辉  夏树伟  赵海洲 《中国海洋湖沼学报》2009,27(2):309-316

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Using otolith weight to predict the age of <Emphasis Type="Italic">Pennahia macrocephalus</Emphasis> in the mouth of the Beibu Gulf     

颜云榕  吴艳芳  卢伙胜  李忠炉  金显仕 《中国海洋湖沼学报》2009,27(2):342-349

The relation between otolith weight (OW) and the age of marine fish is studied. A total of 222 individuals of bighead white croaker, Pennahia macrocephalus were sampled seasonally in the mouth of the Beibu Gulf, the South China Sea, in 2007. Since there are no significant differences in sagittal OW between otolith in pairs (P≥0.05), the undamaged left sagittal otolith is used for age determination. The highest correlations among standard length, OW and fish ages are confirmed by linear, exponential and multinomial regression. Results show that sagittal OW overlaps only occasionally among age groups, and to individuals with similar standard length, the older and slower-growing fish has a heavier otolith because of the continued otolith material deposition. There are differences in sagittal OW among different age groups and significant positive linear relationship with age (P<0.05). The age readings can be verified by plotting the sagittal OW versus the standard length for age groups, and the individuals with similar standard length but in different ages can be separated by sagittal OW frequency analysis. Mostly, the predicted ages using the regression between sagittal OW and ages are closed to the observed ages by counting annulus on scale. It indicates that the sagittal OW analysis is a useful technique for validating the accuracy of age determination by annuli counts, especially for individuals of similar size. Furthermore, the technique is applied for Pennahia macrocephalus with discussion in this paper. Supported by the National Natural Science Foundation of China (No. 30771653) and Bureau of Fisheries, Ministry of Agriculture  相似文献   

Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome   总被引：1，自引：0，他引：1  

闫秀英  孙修勤 《中国海洋湖沼学报》2009,27(2):337-341

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)  相似文献   

Formation and early development of tetraspores of Polysiphonia urceolata （Rhodomelaceae, Rhodophyta）     

姚建亭  李大鹏  于深辉  刘吉东  段德麟 《中国海洋湖沼学报》2009,27(2):365-369

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18°C, 25 μmol photons m⁻² s⁻¹ in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions. Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21), National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06) and Shandong Agricultural Seed Stock Breeding Project  相似文献   

The impact of physical processes on pollutant transport in Hangzhou Bay     

李宁  毛志华  张庆河 《中国海洋湖沼学报》2009,27(2):266-276

A Lagrangian tracer model is set up for Hangzhou Bay based on Coupled Hydrodynamical Ecological model for Regional Shelf Sea (COHERENS). The study area is divided into eight subdomains to identify the dominant physical processes, and the studied periods are March (the dry season) and July (the wet season). The model performance has been first verified by sea-surface elevation and tidal current observations at several stations. Eight tracer experiments are designed and Lagrangian particle tracking is simulated to examine the impact of physical processes (tide, wind and river runoff) on the transport of passive tracer released within the surface layer. Numerical simulations and analysis indicate that: (1) wind does not change the tracer distribution after 30 days except for those released from the south area of the bay during the wet season; (2) the tide and the Qiantang River runoff are important for particle transport in the head area of the bay; (3) the Changjiang River runoff affects the tracer transport at the mouth of the bay, and its impact is smaller in the dry season than in the wet season. Supported by the Natural Science Foundation of China (No.40576080); National High Technology Research and Development Program of China (863 Program, No. 2007AA12Z182)  相似文献   

Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>     

王昌留  张士璀  宿烽  王磊  李红岩 《中国海洋湖沼学报》2009,27(1):69-73

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)  相似文献   

Black carbon and polycyclic aromatic hydrocarbons (PAHs) in surface sediments of China’s marginal seas   总被引：8，自引：0，他引：8  

康延菊  王旭晨  戴民汉  冯桓  李安春  宋茜 《中国海洋湖沼学报》2009,27(2):297-308

This study investigates the distribution of black carbon (BC) and its correlation with total polycyclic aromatic hydrocarbons (ΣPAH) in the surface sediments of China’s marginal seas. BC content ranges from <0.10 to 2.45 mg/g dw (grams dry weight) in the sediments studied, and varied among the different coastal regions. The Bohai Bay sediments had the highest BC contents (average 2.18 mg/g dw), which comprises a significant fraction (27%–41%) of the total organic carbon (TOC) preserved in the sediments. In ...  相似文献   

Diversity and spatial distribution of Opuntia spp. in the Mediterranean Basin     

P. Erre  I. Chessa  G. Nieddu  P.G. Jones   《Journal of Arid Environments》2009,73(12):1058-1066

This work offers a methodology based on statistical tools and GIS applications in a study of the adaptability of Opuntia to the environment, applied on the Mediterranean island of Sardinia. Detailed surveys of habitats containing naturalized populations of Opuntia spp. were made in order to select study sites. The topographic, soil and climatic features of these are described. The local biotypes are ascribed mainly to the spineless Opuntia ficus–indica (L.) Mill. and to the spiny Opuntia amyclaea Ten. Although introduced at different times, for different uses, they have adapted to the Sardinian environment and established in the wild. The distributions of the two species differed. The FloraMap^® modelling approach was used to simulate Opuntia distribution as a function of the island's climate and produced individual distribution maps using the accession points for the two species. Our analysis showed that environmental conditions at elevations above 800 m are the main limiting factors for the presence and abundance of Opuntia in Sardinia. Spatial representation developed by GIS software indicated temperatures below 2 °C as the threshold impairing the species naturalized distribution. The statistic results also show that variation in rainfall does not affect Opuntia spatial distribution in Sardinia.  相似文献   

Effects of diet and body size on phosphorus utilization of Liza haematocheila T. ＆ S.   总被引：1，自引：0，他引：1  

康斌  线薇薇  武云飞 《中国海洋湖沼学报》2008,26(2):157-161

A 21-d laboratory experiment was conducted to study, the phosphorus （P） utilization of two different diets by redlip mullet Liza haematocheila T. ＆ S. Sand-filtered water in salinity 30 and temperatare 25℃ was used. Twenty-nine fish individuals were divided into three groups： 11 to group 1 （G1） fed on diet 1, 11 to group 2 （G2） fed on diet 2, and 7 to contrast group. Diet 1 was a commercial feed, more valuable in nutrition than diet 2 that similar to natural detritus. The results show the intake phosphorus （IP） of G1 was significantly higher than that of G2, and both increased linearly with body size at a certain amount of diet. The retention phosphorus （RP） in fish of G1 was lower than G2. The relationship between retention phosphorus and body size was positive and stronger in G2. Significant difference in faecal phosphorus （FP） was found between G1 and G2. Body size significantly impacted the excretion phosphorus （EP） in G1 but G2. The loss of intake phosphorus in G1 was 10.83-20.27 mg per g fish weight gain, higher than that in G2 for 6.63-9.56. Of the phosphorus, about 10% was allocated into growth, 50% in faeces, and the rest lost in excretion. The main part of phosphorus was lost in faeces but excretion. The phosphorus budget of the fish could be described as 100IP = 7.40RP ＋ 47.39FP ＋ 36.63EP （Diet 1） or 100IP = 11.93RP ＋ 56.64FP ＋ 21.76EP （Diet 2）.  相似文献   

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone <Emphasis Type="Italic">Haliotis diversicolor supertexta</Emphasis>     

张祥景  杨志会  蔡中华 《中国海洋湖沼学报》2008,26(3):238-241

Abalone Haliotis diversicolor supertexta is an important economic mollusk. The settlement and metamorphosis are two critical stages during its development period, which has direct influence on abalone survival and production. The influence of reactive oxygen species （hydrogen peroxide） on abalone embryo and juvenile development were examined in this study. Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide. They had the best performance at 800 μmol/L. The concentration of 1 000 μmol/L or higher was toxic to the larvae, as the larvae could settle down only at benthic diatom plates without complete metamorphosis. In addition, H2O2 adding time was critical to the larval performance. 24h after two-day post-fertilization was proved to be the optimal adding time. In this paper, two action mechanisms of hydrogen peroxide are discussed： （1） hydrogen peroxide has direct toxicity to ciliated cells, thus cause apoptosis; （2） hydrogen peroxide, as a product from catecholamines＇ autoxidation process in vivo, can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.  相似文献